Construction and Application of Nucleic Acids-Based Biomolecular Condensates

and intracellular biomolecules (adenosylmethionine and tetracycline), as well as target cells with overexpressed epithelial cell adhesion molecules. In addition, we leveraged the strong Watson-Crick base pairing ability to recruit the intracellular target RNA into condensates for cellular regulation.In this Account, we give an overview of nucleic acid-based biomolecular condensates. We first discuss the intermolecular interactions and forces involved in the formation of nucleic acid-based biomolecular condensates. Subsequently, we summarize recent research about nucleic acid-based condensates and their applications in the fields of biological imaging and biosensing, cell simulation, cellular regulation, and drug delivery. Finally, we outline the current challenges and future opportunities of nucleic acid-based biomolecular condensates. We hope that this Account will afford significant inspiration in the design of nucleic acid-based condensates and the applications in cell biology and biomedicines.