Novel Green Spectrofluorimetric‐Derived Quenching Assay for the Efficient Determination of 3‐Chlorotyrosine Marker of Myeloperoxidase‐Catalyzed Protein Oxidation in Pure Form and Biological Fluids

420 nm), in the presence of sodium hydroxide (2 mM) and 1.54-mM cetyl tetraammonium bromide (CTAB), is decreased linearly by the addition of Cl-Tyr in the range from 0.1 to 15 μg/mL with a correlation coefficient of 0.9988, and the detection limit was 0.044-μg/mL Cl-Tyr. The intraday and interday percent recoveries ranged from 95.392% to 99.280%, with a percent relative standard deviation (%RSD) of 0.332%-2.128%, indicating that the developed method is both accurate and precise for the determination of Cl-Tyr. Moreover, the proposed method was successfully applied to determine Cl-Tyr in human serum samples, with recovery percentages ranging from 94.842% to 101.192%, confirming its reliability and applicability to biological matrices. Accordingly, the 2-naphtholate/CTAB fluorescent platform offers an inexpensive, accessible, and environmentally considerate alternative to other sophisticated and time-consuming technicalities utilized for routine Cl-Tyr monitoring.