Genomic Characterization of Antibiotic-Resistant Enterococcus Spp. Strains in Clinical Settings of Yichang, China

Objective: species are important causes of hospital-acquired infections, yet genomic surveillance data from Yichang, China, are lacking. Methods: isolates were collected from five hospitals in Yichang. Antimicrobial susceptibility testing was performed according to CLSI standards. Fifty-nine representative isolates underwent whole-genome sequencing for species identification, multilocus sequence typing (MLST), antimicrobial resistance (AMR) gene detection, virulence profiling, and phylogenetic analysis. Results: . Conclusion: species. The detection of two novel sequence types and region-specific resistance patterns highlights the value of genomic monitoring in guiding infection-control policies, antimicrobial stewardship, and early warning of multidrug-resistant strain dissemination in hospital settings.