Meniscus‐Mediated Imaging of Soft Biological Membranes Using 200 kN/m Ultra‐Stiff 15 MHz <scp>MEMS AFM</scp> Probes

and a resonance frequency of 15 MHz, can image soft cell membranes of Halobacterium Sallinarium in ambient air without structural disruption. Although meniscus-mediated tip stabilization has been previously observed for MEMS and optomechanical probes, this study provides the first demonstration of its application to biological imaging. By operating in a capillary-coupled regime with sub-ångström oscillation amplitude, the probe stabilizes at approximately 40% of the meniscus rupture distance, dissipating only ~35% of the maximum interaction energy. This enables noncontact imaging at tip-sample separations of ~5 nm and faithful recovery of the membrane topography (~5 nm thickness). The results establish a pathway for ultra-stiff AFM probe to perform noninvasive imaging of soft biological structures in air.