PREDICTOR: A Non‐Enzymatic Catalytic Cascade Tool for in Situ Visualization of Small Extracellular Vesicle Surface glycoRNAs

= 0.9816 vs. 3.495 and 2.711). Applying PREDICTOR to a breast cancer transformation model (MCF-10A → MCF-7 → MDA-MB-231) showed that multiple surface glycoRNA candidates decrease with malignancy, accompanied by progressive softening of sEV membranes (Young's modulus reduced by 25.08% and 54.72%). Functionally, enzymatic depletion of surface RNA and disruption of sialylated N-glycan features attenuated macrophage uptake and inflammatory activation, supporting a contribution of surface glycoRNAs to sEV recognition. Collectively, PREDICTOR provides a rapid, enzyme-free platform for quantitative and spatial profiling of sEV surface glycoRNAs and links their abundance to vesicle mechanics and immune-cell interactions.