Validation of a Duplex Digital PCR Assay for the Quantification of the NK603 Maize Event Across Three dPCR Platforms
validation of a duplex dPCR assay targeting the maize GM event NK603 and the HMG reference gene was performed on three platforms: Bio-Rad QX200™ (Pleasanton, CA, USA), Qiagen QIAcuity (Venlo, The Netherlands), and Thermo Fisher QuantStudio Absolute Q (Waltham, MA, USA). All validation parameters met the Joint Research Centre (JRC) acceptance criteria. In particular, this assay demonstrated high specificity, sensitivity (limit of quantification or LOQ < 35 copies per reaction), precision, and trueness (RSDr and bias <25%). The data indicate that the duplex dPCR assay can be used for routine GMO analysis and future collaborative validation studies.
Authors
- Ugo Marchesi (ORCID: https://orcid.org/0000-0002-7789-241X)
- Elisa Pierboni (ORCID: https://orcid.org/0000-0003-3488-5743)
- Silvana Farneti (ORCID: https://orcid.org/0000-0002-1408-8838)
- Davide La Rocca (ORCID: https://orcid.org/0000-0001-9456-359X)
- Katia Spinella (ORCID: https://orcid.org/0000-0003-3839-7974)
- Sara Ciuffa (ORCID: https://orcid.org/0000-0003-1739-3595)
- Raffaele Carrano
- Daniela Verginelli (ORCID: https://orcid.org/0000-0001-9247-7073)
- Monica Borghi (ORCID: https://orcid.org/0000-0002-6337-5999)
Institutions
- Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche (IT)
- Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana (IT)
Publication Details
- Journal
- Foods
- Published
- 2026-04-14
- DOI
- https://doi.org/10.3390/foods15081366
- Primary Topic
- Genetically Modified Organisms Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00