Rationally Designing a Fluorogenic Substrate of HRP To Bring Benefits for Enzyme Cascade-based Fluorescence-Activated Droplet Sorting
and greatly elevated anti-interference capability in comparison with ADHP. In the present study, the advantages of AQHR were confirmed by multienzyme cascade fluorescence assays and FADS screening of carboxylesterase (CES) mutants. AQHR is expected to become a promising substrate for HRP and be widely used in FADS and other analytical fields.
Authors
- Tao Deng (ORCID: https://orcid.org/0000-0002-3965-9553)
- Fuqiang Ma (ORCID: https://orcid.org/0000-0001-5614-6496)
- Fang Liu (ORCID: https://orcid.org/0000-0003-1707-5183)
- Xiaotian Hu
- Xiao Li
- Guilin Li
Institutions
- Guangzhou University of Chinese Medicine (CN)
- Ministry of Education of the People's Republic of China (CN)
- Foshan University (CN)
- Suzhou Institute of Biomedical Engineering and Technology (CN)
- Indiana University School of Medicine
Publication Details
- Journal
- Analytical Chemistry
- Published
- 2026-04-14
- DOI
- https://doi.org/10.1021/acs.analchem.6c00337
- Primary Topic
- Innovative Microfluidic and Catalytic Techniques Innovation
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Youth Innovation Promotion Association of the Chinese Academy of Sciences
- Jiangsu Provincial Key Research and Development Program
- National Science and Technology Major Project
- Basic and Applied Basic Research Foundation of Guangdong Province