Protein-Synthetic Activity of White Rat Pulmonary Fibroblasts under Oxidative Stress

Objective: Proline-containing peptides have a wide range of biological effects, including antioxidant ones. The effect of glyprolines on the proteinsynthetic activity of cells was studied using a model of oxidative stress in a primary culture of pulmonary fibroblasts from newborn white rats. The aim of the study was to investigate the effect of regulatory peptides of the glyproline series on the primary culture of white rat pulmonary fibroblasts under normal conditions and under oxidative stress. Material and methods: Peptide solutions at various doses were applied to monolayers of primary fibroblast cultures from neonatal white Wistar rats using a model of oxidative stress induced by H2O2. Results: After the introduction of glyprolines into the culture medium, an increase in nucleolar area was recorded, which positively correlated with an increase in proteinsynthetic activity. When oxidative stress was modelled in the pulmonary fibroblast culture, a decrease in nucleolar area was observed. All peptides (ProGlyProVal at doses of 50, 100 and 500 µg/L; ArgProGlyPro and ProGlyProLeu at a dose of 100 µg/L; ThrLysProArgProGlyPro at doses of 100 and 1000 µg/L) offset the inhibitory effect on protein synthesis after oxidative stress to varying degrees. Conclusions: All of the studied glyprolines have protective properties at the cellular level in vitro.