In Vitro Antiviral Properties of Two Recombinant Sendai Virus Vectors Encoding ORFV 011 and ORFV 059 Genes
was robustly upregulated, suggesting a potential role in restricting ORFV replication. Antiviral activity assays revealed a marked reduction in ORFV DNA copies and a mild decrease in ORFV RNA transcription in rSeV-GFP-059-transduced cells, particularly at later time points, accompanied by complete abrogation of the typical cytopathic effect. Collectively, these results demonstrate that SeV-based vectors, particularly rSeV-GFP-059, efficiently prime antiviral immunity and suppress ORFV replication, establishing a promising platform for further in vivo vaccine evaluation in sheep.
Authors
- Álex Gómez (ORCID: https://orcid.org/0000-0002-9723-9004)
- L. Luján
- Mikel Nazabal
- L. Puzol
- Idoia Glaria (ORCID: https://orcid.org/0000-0003-4556-489X)
- Irache Echeverría (ORCID: https://orcid.org/0000-0003-2399-0601)
- I. Moncayola
- I. Hualde
- Ralf Amann (ORCID: https://orcid.org/0009-0005-8482-2240)
- Ramsés Reina (ORCID: https://orcid.org/0000-0003-1265-9139)
- Benhur Lee
- Ainhoa Calero
- Laura Arriazu
- Ignacio de Blas
Institutions
- Universidad Pública de Navarra (UPNA) (ES)
- Universidad de Zaragoza (ES)
- Hospital Universitario Miguel Servet (ES)
- Centro de Investigación y Tecnología Agroalimentaria de Aragón (ES)
- Gobierno de Navarra (ES)
- Instituto Navarro de Tecnología e Infraestructuras Agroalimentarias (ES)
- Agrobiotechnology Institute (ES)
- University of Tübingen (DE)
- Icahn School of Medicine at Mount Sinai (US)
Publication Details
- Journal
- Viruses
- Published
- 2026-04-13
- DOI
- https://doi.org/10.3390/v18040462
- Primary Topic
- Poxvirus research and outbreaks
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Gobierno de Navarra