Three-dimensional Bovine Collagen–Elastin Matrix in Soft Tissue Reconstruction: No Detectable Bovine DNA After 1 Year

Background: Postoperative wound healing remains a critical challenge, particularly in complex reconstructions such as free flap surgeries. Despite technical improvements, perfusion-related complications may cause partial flap loss. Following wound stabilization, secondary measures such as vacuum-assisted closure therapy and skin grafting are required. Three-dimensional bovine collagen-elastin matrices offer promising support by enhancing cell migration, angiogenesis, and tissue regeneration. Methods: We report the case of a 43-year-old woman who developed partial necrosis after deep inferior epigastric perforator flap reconstruction. She was treated with vacuum-assisted closure therapy, followed by the application of MatriDerm 1 mm and split-thickness skin grafting. After 12 months, histological analysis and quantitative reverse transcription polymerase chain reaction (PCR) were performed on the grafted area to assess tissue integration and detect residual bovine DNA. Results: Histology showed full regeneration of the epidermis and dermis without detectable bovine matrix remnants. No elastic fibers from MatriDerm were visible, and quantitative reverse transcription PCR confirmed the absence of bovine DNA, despite ongoing tissue remodeling and macrophage infiltration. Conclusions: These findings demonstrate complete resorption and integration of a 3-dimensional bovine collagen-elastin matrix within 1 year, without immunologically relevant residues. Compared with other dermal substitutes such as Integra, MatriDerm shows faster degradation and a lower risk of foreign body reactions. The observed tissue remodeling indicates a prolonged regenerative process depending on wound type and depth. MatriDerm is a safe and effective dermal matrix, ensuring complete resorption and tissue integration. The absence of bovine DNA supports its immunologic safety and clinical suitability for complex wound treatment requiring durable and aesthetic outcomes.