Biotin-Decorated Inulin-Based Polymeric Micelles Unveil Their Dual-Targeting Ability for the Potential Treatment of Glioblastoma Multiforme through the <i>In Vitro</i> and <i>In Vivo</i> Investigations

biological studies on GBM U87MG cells indicated that empty micellar carriers did not reveal any significant cytotoxicity and that the encapsulation of curcumin within the carrier can enhance the curcumin's efficacy, potentially by improving its bioavailability. Furthermore, confocal microscopy and flow cytometry studies revealed that the presence of biotin moieties is pivotal to enhancing the cellular uptake and retention of the INVITE nanosystems. In conclusion, INVITE-BIO micelles enhanced both the crossing of BBB and drug accumulation in GBM tumor cells overexpressing the receptor for biotin through receptor-mediated endocytosis, demonstrating the great potential of biotinylated INVITE micelles as a promising dual-targeted approach for i.v. administration of antitumoral drugs for the treatment of Glioblastoma Multiforme.