Histotripsy treatment reduces tumor burden and extends survival in an orthotopic mouse model of osteosarcoma

Background: Osteosarcoma (OS) is a devastating primary bone tumor. Histotripsy, a non-invasive ablation modality that mechanically destroys tissue with focused ultrasound, has shown promise for the treatment of OS. The goal of this study is to characterize the ablative effects after histotripsy treatment in an orthotopic mouse model of OS. Methods: ). Histotripsy outcomes were evaluated and compared on MRI and histopathology at different acute (1 and 3) and sub-acute (7 and 14) days post-treatment (DPT). 5 mice were survived up to 30 DPT, and factors correlating with survival were evaluated. Results: < 0.0001) by a median of 15 days compared to untreated OS mice. Conclusions: Histotripsy treatment significantly reduced tumor burden and extended survival compared to untreated controls. These results highlight histotripsy's promise as a safe and effective non-invasive treatment for OS.