Double-helix optical point spread function enables real-time mesoscopic 3D functional microangiography in the living mouse brain and skull
volume. Beyond high-resolution structural imaging, perfusion time-to-peak values are extracted from the laser-scanning configuration while accurate flow velocity/direction information is provided via widefield tracking of fluorescently labeled cells. We demonstrate the platform's capabilities by analyzing brain-layer-specific perfusion dynamics and vascular topology in glioma-bearing mouse brains, offering unprecedented views for probing cerebrovascular alterations in both physiological and pathological contexts.
Authors
- Qiang Fu (ORCID: https://orcid.org/0000-0001-6395-8521)
- Xuyang Chang (ORCID: https://orcid.org/0000-0001-5696-0033)
- Bruno Weber (ORCID: https://orcid.org/0000-0002-9089-0689)
- Dominik Schillinger (ORCID: https://orcid.org/0000-0002-9068-6311)
- Zhenyue Chen (ORCID: https://orcid.org/0000-0001-9508-7521)
- Lin Tang (ORCID: https://orcid.org/0009-0008-3399-1134)
- Quanyu Zhou (ORCID: https://orcid.org/0000-0001-7719-4649)
- Etienne Jessen (ORCID: https://orcid.org/0000-0003-0276-6029)
- Chaim Glück (ORCID: https://orcid.org/0000-0002-8754-9965)
- Xosé Luís Deán‐Ben (ORCID: https://orcid.org/0000-0002-8557-7778)
- Lukas Glandorf (ORCID: https://orcid.org/0000-0003-4968-2906)
- Wolfgang Heidrich (ORCID: https://orcid.org/0000-0002-4227-8508)
- Daniel Razansky (ORCID: https://orcid.org/0000-0001-8676-0964)
- Hadi Amata (ORCID: https://orcid.org/0000-0002-9343-729X)
- Michael Reiss (ORCID: https://orcid.org/0000-0003-3583-3451)
- Xiong Dun
- Shiyao Guo (ORCID: https://orcid.org/0000-0002-6362-5732)
- Tian Jin
- Baoyuan Zhang
- Weibo Wang
- Shuxin Lyu
Institutions
- Tongji University (CN)
- University of Zurich (CH)
- Harbin Institute of Technology (CN)
- Technische Universität Darmstadt (DE)
- ETH Zurich (CH)
- Institute for Biomedical Engineering (CH)
- King Abdullah University of Science and Technology (SA)
Publication Details
- Journal
- Nature Communications
- Published
- 2026-04-13
- DOI
- https://doi.org/10.1038/s41467-026-71746-9
- Primary Topic
- Advanced Fluorescence Microscopy Techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Science Foundation
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
- National Natural Science Foundation of China
- Fundamental Research Funds for the Central Universities