Sequential Electrospinning of Asymmetric PDLLA/PVP-HA Scaffolds Functionalized with Glycine for Medical Devices

In this study we engineered bilayered electrospun scaffolds consisting of a hydrophobic PDLLA and hydrophilic PVP layer that incorporate either native HA or semi-synthetic HA-Gly-OH at concentrations of 1% and 3% w/w. Generally, bilayer scaffolds electrospun on different days delaminated, while herein they maintained their integrity because they were electrospun on the same day. Sequential electrospinning enabled the bilayer structure characterized via Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM), and Young’s modulus measurements to assess morphology and mechanics. In vitro cytotoxicity and cell viability assays with fibroblast cells confirmed good biocompatibility for both the individual layers and the bilayer system. Among the tested formulations, the bilayer PDLLA/PVP–HA-Gly-OH 1% showed the most promising performance, attributed to the synergistic effects of HA and Gly-OH in promoting adhesion and proliferation.

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Publication Details

Journal
Polysaccharides
Published
2026-04-13
DOI
https://doi.org/10.3390/polysaccharides7020046
Primary Topic
Electrospun Nanofibers in Biomedical Applications
Type
article
Field-Weighted Citation Impact
0.00

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article

Sequential Electrospinning of Asymmetric PDLLA/PVP-HA Scaffolds Functionalized with Glycine for Medical Devices

Brigida Bochicchio, Antonietta Pepe, Katja Schenke‐Layland, Grazia M. L. Messina et al.
Polysaccharides
Electrospun Nanofibers in Biomedical Applications
article

Sequential Electrospinning of Asymmetric PDLLA/PVP-HA Scaffolds Functionalized with Glycine for Medical Devices

Brigida Bochicchio, Antonietta Pepe, Katja Schenke‐Layland, Grazia M. L. Messina, Luigi Fabiano, Giovanna Calabrese, Antonio Laezza, Ileana Ielo, Giovanna De Luca, Francesca Armiento, Paola Campione, Serena Munaò, Matteo Carrozzino
article en

Abstract

In this study we engineered bilayered electrospun scaffolds consisting of a hydrophobic PDLLA and hydrophilic PVP layer that incorporate either native HA or semi-synthetic HA-Gly-OH at concentrations of 1% and 3% w/w. Generally, bilayer scaffolds electrospun on different days delaminated, while herein they maintained their integrity because they were electrospun on the same day. Sequential electrospinning enabled the bilayer structure characterized via Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM), and Young’s modulus measurements to assess morphology and mechanics. In vitro cytotoxicity and cell viability assays with fibroblast cells confirmed good biocompatibility for both the individual layers and the bilayer system. Among the tested formulations, the bilayer PDLLA/PVP–HA-Gly-OH 1% showed the most promising performance, attributed to the synergistic effects of HA and Gly-OH in promoting adhesion and proliferation.

PolysaccharidesVol. 7(2)
University of Messina (IT), Institute for Women's Policy Research (US), University of Basilicata (IT), University of Catania (IT), Natural and Medical Sciences Institute (DE)
European Commission, Sapienza Università di Roma, Università degli Studi della Basilicata
Openalex Percentile: Top 19%
Electrospun Nanofibers in Biomedical Applications
DOIOpen Access PDFOpenAlex
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