In Vitro Analysis of Gene and Protein Expression in Primary Limbal Epithelial Cells Exposed to Differentiation-Inducing Medium
CnT-2D differentiation medium effectively initiates differentiation of limbal epithelial cells in vitro. The gradual increase in the expression of key differentiation markers, including DSG1, KRT3, and PAX6, indicates that CnT-2D medium successfully induces differentiation in 2D cultured primary limbal epithelial cells. However, subcellular localization of these markers, epithelial barrier function, and differentiation in 3D models were not assessed and remain to be investigated.
Authors
- Priya Katiyar
- Shweta Suiwal (ORCID: https://orcid.org/0000-0002-1230-1279)
- Nóra Szentmáry (ORCID: https://orcid.org/0000-0001-8019-1481)
- Fabian Norbert Fries (ORCID: https://orcid.org/0000-0002-8526-4712)
- Shanhe Liu
- S. L. Hsu
- Shuailin Li (ORCID: https://orcid.org/0009-0007-2251-6242)
- Maryam Amini
- Swarnali Kundu
- Virendra Kumar
- Tanja Stachon
- Berthold Seitz (ORCID: https://orcid.org/0000-0001-9701-8204)
Institutions
- Sandip Foundation (IN)
- Saarland University (DE)
Publication Details
- Journal
- Biology
- Published
- 2026-04-12
- DOI
- https://doi.org/10.3390/biology15080610
- Primary Topic
- Corneal Surgery and Treatments
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- China Scholarship Council
- Dr. Rolf M. Schwiete Stiftung