Split-crRNA Transcription Based Dual-Recognition Cascade Amplification Assay for Sensitive and Accurate Postoperative Delirium Related MicroRNA Analysis

-cleavage, the assay achieves exceptional sensitivity, with a detection limit as low as 0.52 fM for miRNA-193 and a wide linear range from 1 fM to 100 pM. It demonstrates high specificity by effectively discriminating single-base mismatches, and performs robustly in spiked human serum, yielding recovery rates of 97.3-107.2%. Moreover, results correlate closely with RT-qPCR, confirming reliability in complex matrices. This work presents a versatile and robust biosensing platform that not only offers a promising tool for POD-related miRNA analysis, but also establishes a modular framework adaptable for detecting diverse nucleic acid targets in point-of-care diagnostics.