Systematic CRISPRi screening reveals genetic modulators of <i>E. coli</i> isoprenoid production

genes and found 31 genes for which repression significantly modified lycopene yield. Genes whose repression increased lycopene yield were found across fatty acid, amino acid, central carbon, and isoprenoid metabolism as well as stress response pathways. This set included four genes in amino acid biosynthesis, one in phospholipid biosynthesis, and one in the stringent response that, to our knowledge, have not previously been implicated in lycopene production. In contrast, genes for which repression decreased yield were limited to isoprenoid biosynthesis, central carbon metabolism, and stress response pathways. Together, our work reveals genetic targets for increasing lycopene yield throughout metabolism, and defines a tractable, generalizable approach to mapping genetic factors that modulate biosynthetic pathway yield.