Hypsizygus marmoreus polysaccharides protect against cisplatin-induced intestinal mucositis via modulation of gut microbiota, inflammation, and intestinal barrier function

Introduction: polysaccharides (HMP) against cisplatin-induced intestinal mucositis in mice. Methods: radical-scavenging assay. A cisplatin-induced mucositis mouse model was established to assess the protective effects of HMP on clinical symptoms, intestinal histopathology, inflammatory cytokines, epithelial barrier integrity, and gut microbiota composition using 16S rRNA sequencing. Results: were enriched following HMP administration. Discussion: These findings indicate that HMP protects against cisplatin-induced intestinal mucositis through multiple complementary mechanisms, including antioxidant activity, suppression of inflammatory responses, preservation of epithelial barrier integrity, and modulation of gut microbiota composition. HMP therefore represents a promising natural candidate for mitigating chemotherapy-induced gastrointestinal toxicity.