An Automated AI-Based Vision Inspection System for Bee Mite and Deformed Bee Detection Using YOLO Models

Varroa destructor (Bee mite) and Deformed Wing Virus are primary causes of honeybee colony collapse. This study developed an automated AI-based vision inspection system for detecting bee mites and deformed bees using the YOLO algorithm. The system integrates an RGB camera, a beecomb rotation motor, and an image transmission module to enable automated dual-sided image acquisition of the beecomb. The image characteristics of normal bees, bee mites, and deformed bees were analyzed, and YOLO-based object detection models were developed to classify them. Six YOLO models—based on YOLOv8 and YOLOv11 architectures across three model sizes (nano, small, and large)—were evaluated on 405 test images (6441 objects). The proposed system reduced the inspection time from 240 s required for manual method to 20 s per beecomb, achieving 12-fold efficiency improvement. Comparative analysis showed model-task specialization: YOLOv8l excelled in detecting small bee mites (F1: 92.5%, mAP[0.5]: 92.1%), while YOLOv11s achieved the highest performance for morphologically diverse deformed bees (F1: 95.1%). Error analysis indicated that detection performance was influenced by morphological characteristics. Deformed bee detection errors correlated with overlap in wing-to-body ratio: DB Type II exhibited 18.6% miss rate, while DB Type III achieved perfect detection. In bee mite detection, a sensitivity–specificity trade-off was observed: YOLOv11l had the lowest false negatives (2.5%) but highest false positives, while YOLOv8l demonstrated superior discrimination. These results demonstrate the practical potential of the proposed system for field deployment in apiaries, supporting early pest diagnosis and improved colony health management. The model-task specialization framework provides guidance for architecture selection based on object characteristics. Future work will focus on multi-location validation and real-time monitoring integration.