Engineering an <scp>l</scp> -Threonine Aldolase from <i>Staphylococcus epidermidis</i> for Enhanced Diastereoselectivity in the Synthesis of a Chloramphenicol Intermediate

. Furthermore, Mut4 exhibits markedly improved diastereoselectivity toward para- and meta-substituted benzaldehyde derivatives. Molecular dynamics (MD) simulations further elucidated the molecular basis underlying the enhanced diastereoselectivity of Mut4. This study provides a potential biocatalyst for the sustainable and efficient synthesis of a chloramphenicol intermediate.