Evaluation of a New Automated Platform for the Detection and Quantification of HEV RNA
IU/mL. All HEV RNA were detected in stool samples with both methods. These results indicated a lack of sensitivity for the ELITe assay. A second protocol using 600 µL of sample eluted in 50 µL of solution was evaluated and presented a limit of detection of 69 IU/mL using the WHO standard. While the ELITe assay demonstrated good linearity and correlation with the AltoStar® assay, it struggled to detect low levels of HEV RNA. However, a new extraction protocol may help to overcome this drawback but needs further validation and changes in the package insert of the manufacturer.
Authors
- Florence Abravanel (ORCID: https://orcid.org/0000-0002-1753-1065)
- Benoît Visseaux (ORCID: https://orcid.org/0000-0002-9279-5538)
- Céline Delevallee
- Sébastien Lhomme (ORCID: https://orcid.org/0000-0001-7908-1576)
- Xavier Huc
- Stéphanie Haïm‐Boukobza
- Aurélie Guigon
- Annlyse Esteban
- Soheil Elhayani
- Jacques Izopet
- Mélanie Lecrac
Institutions
- Saint-Gobain (France) (FR)
- Centre National de la Recherche Scientifique (FR)
- Inserm (FR)
- Université Fédérale de Toulouse Midi-Pyrénées (FR)
- Centre Hospitalier Universitaire de Lille (FR)
- University of Clermont Auvergne (FR)
- Laboratoire National de Référence (MA)
- Centre de Physiopathologie de Toulouse-Purpan (FR)
- Hôpital Purpan (FR)
Publication Details
- Journal
- Journal of Medical Virology
- Published
- 2026-04-01
- DOI
- https://doi.org/10.1002/jmv.70923
- Primary Topic
- Hepatitis Viruses Studies and Epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 0.00