Tailored analytical strategies for AAV aggregation: purpose‑driven method selection for enhanced product development
No single analytical method captures all AAV aggregation species across all conditions. A purpose-driven framework for selecting SEC-UV, SEC-FLD, DLS, and A4F – what each method detects, where each fails, and how to combine them for release testing, stability studies, and forced degradation.
Authors
- Nina Liu (ORCID: https://orcid.org/0009-0005-6910-8823)
- Aishwarya Shevade
- Dingjiang Liu (ORCID: https://orcid.org/0000-0002-8665-2426)
- Yuan Cao (ORCID: https://orcid.org/0000-0002-4632-9990)
- Lin Wang (ORCID: https://orcid.org/0009-0005-7958-9894)
- Michael Rosconi (ORCID: https://orcid.org/0009-0004-2285-2164)
- Polat Abdubek
- Andrew D. Tustian (ORCID: https://orcid.org/0000-0003-2860-5506)
- Lin Luo (ORCID: https://orcid.org/0009-0003-8055-546X)
- Serena Wang
- John S Reeves
- Mohammed Shameem
- Yan Zhao
- Yan Yu
- Kenneth Graham
- Glynn O’Grady
- Wen Shi
Institutions
- Regeneron (United States) (US)
Publication Details
- Journal
- Cell and Gene Therapy Insights
- Published
- 2026-03-31
- DOI
- https://doi.org/10.18609/cgti.2026.029
- Primary Topic
- Virus-based gene therapy research
- Type
- article
- Field-Weighted Citation Impact
- 0.00