In Vitro Evaluation of the Synergistic Acid-Neutralizing and Pitta-Balancing Effects of Ayurvedic Nutritional Herbs Extract: Cuminum cyminum, Foeniculum vulgare, and Phyllanthu semblica

The present study investigated the in vitro synergistic acid-neutralizing and Pitta-balancing potential of  hydroalcoholic extracts of Cuminum cyminum, Foeniculum vulgare, and Phyllanthus emblica. Individual extracts and their combined formulation (1:1:1) were evaluated for acid-neutralizing capacity, buffering capacity, simulated gastric fluid neutralization, total phenolic content, and antioxidant activity using the DPPH assay. The polyherbal formulation demonstrated superior acid-neutralizing capacity compared to individual extracts and exhibited enhanced buffering action by maintaining higher pH levels upon incremental acid addition. In the simulated gastric fluid model, the combined extract significantly elevated gastric pH, indicating improved acid resistance. Total phenolic content was highest in the combined formulation, correlating with increased antioxidant activity. The DPPH radical scavenging assay confirmed concentration-dependent antioxidant effects, with the polyherbal blend showing the greatest inhibition. The findings suggested a synergistic interaction among phytoconstituents such as phenolics, flavonoids, and terpenoids, contributing to enhanced gastroprotective potential. Overall, the study supported the traditional Ayurvedic rationale of combining digestive herbs for effective acid regulation and Pitta balance under in vitro conditions.