Preparation and validation of platelet rich plasma from goat blood using a double centrifugation protocol

The development of species-specific protocols for platelet-rich plasma (PRP) preparation is critical for ensuring quality and reproducibility in veterinary regenerative medicine. This study aimed to standardise the preparation of homologous PRP from goat blood using a double centrifugation method and to validate its efficacy based on platelet enrichment and recovery. Venous blood was collected from bucks and baseline haematological parameters were assessed. A two-step centrifugation protocol was employed: a soft spin at 300g for 5 min. followed by a hard spin at 700g for 17 min. The resulting PRP was analysed for erythrocyte, leukocyte and platelet concentrations, and quantitative indices including platelet enrichment factor (PEF) and platelet recovery rate (PRR) were calculated. Compared with whole blood, PRP exhibited a marked higher concentration of platelet (1465.50 124.42 10³μL vs. 413.50 24.95 10³μL), with an average PEF of 3.74 0.21 and PRR of 78.49 3.94 per cent. Red and white blood cells were substantially reduced in PRP, confirming successful separation. The protocol provided effective platelet concentration while minimising contamination, although separation challenges due to the small size of goat platelets and erythrocytes were noted. These findings support the feasibility of standardised homologous PRP preparation in goats and highlight the need for further refinement to improve purity.