EVALUATION OF ADDING DIFFERENT CONSENTRATIONS FROM CO-ENZYME Q10 ON IMMUNIZATION WITH CLASSICAL AND RECOMBINANT VACCINE AGAINST INFECTIOUS BURSAL DISEASE IN BROILERS

This study was aimed to detect the effect of co-enzyme Q10 against infectious bursal disease vaccinated with different programs (recombinant HVT-IBD single dose at one day old, classical vaccines D78 at eight days old, and E228 at 16 days old) through the evaluation of levels of interferon-gamma (INF-γ) and tumor necrosis factor-alpha (TNF-α) in serum. Two hundred and ten broilers were divided into seven groups, with 30 chicks in each group. T1, feed Q10 (20 mg/kg diet) and vaccinated by (D78, E288); T2: feed Q10 (20 mg/kg diet) and vaccinated by (rHVT); T3: feed Q10 (40 mg/kg diet) and vaccinated by (D78, E288); T4; feed Q10 (40 mg/kg) and vaccinated by (rHVT): T5; vaccinated (D78, E288) only: T6; vaccinated (rHVT) only: T7; negative control. The results of the titers of interferon-gamma (INF-γ) on day 15 showed that T6 and T5 were significantly (P≤0.05) higher compared with other groups. In contrast, on days 25 and 33, they showed a decline throughout the experiment. At the same time, tumor necrosis factor-alpha (TNF-α) results indicated that T1 and T7 recorded a lower level than other groups at day 11. In comparison, on days 19 and 25, T1, T2, and T7 had the lowest titers among the different groups. In conclusion, the co-enzyme Q10 (40 mg/kg feed) enhances immunity function by modulating the immune response, especially (INF-γ), and decreasing pro-inflammatory markers (TNF-α).