Unraveling acridine degradation mechanisms in PAH-contaminated soils using DNA-SIP combined with metagenomics and soil transcriptomics
C-enriched DNA from soil incubations identified a member of the genus Sphingobium as the primary acridine degrader. Transcriptomic analysis based on its 16S rRNA gene expression demonstrated a strong correlation with acridine removal from the soil. Shotgun metagenomic sequencing enabled the reconstruction of one metagenome-assembled genome (MAG). Functional annotation of this MAG revealed five gene clusters potentially involved in acridine biodegradation, and their actual contribution was assessed by gene expression analysis in soil incubations. Based on these findings, we reconstructed the metabolic pathway for putative acridine degradation in PAH-contaminated soil.
Authors
- Joaquim Vila
- Magdalena Grifoll (ORCID: https://orcid.org/0000-0002-5762-4194)
- Juan F. Gago (ORCID: https://orcid.org/0000-0002-0572-1208)
- Zhenyu Tian (ORCID: https://orcid.org/0000-0002-7491-7028)
- David R. Singleton (ORCID: https://orcid.org/0000-0001-6058-6742)
- Esteban Bustos-Caparros (ORCID: https://orcid.org/0000-0002-9489-4441)
- Maria C. Jordan (ORCID: https://orcid.org/0000-0001-5272-2185)
- Zhenfa Zhang (ORCID: https://orcid.org/0000-0002-2672-4416)
- Ramon Rosselló-Móra
Institutions
- University of North Carolina at Chapel Hill (US)
- Clínica Diagonal (ES)
- Mediterranean Institute for Advanced Studies (ES)
- Universitat de Barcelona (ES)
Publication Details
- Journal
- Journal of Hazardous Materials
- Published
- 2026-04-07
- DOI
- https://doi.org/10.1016/j.jhazmat.2026.142004
- Primary Topic
- Microbial bioremediation and biosurfactants
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Ministerio de Ciencia, Innovación y Universidades
- Generalitat de Catalunya
- Universitat de Barcelona
- European Social Fund