Gut microbiota and bile acids profiles study of ulcerative colitis and Crohn’s disease patients

Introduction: The global incidence of inflammatory bowel disease (IBD) is increasing, with dysbiosis of the gut microbiota identified as a significant factor contributing to chronic intestinal inflammation. This study aims to elucidate the gut microbiota and serum bile acid profiles in patients with ulcerative colitis (UC) and Crohn's disease (CD), and to explore the relationship between bacterial taxa and bile acids. Methods: We recruited 31 UC patients, 41 CD patients, and 32 healthy controls from The First Affiliated Hospital of Zhejiang Chinese Medical University. Gut microbiota composition was analyzed by 16S rDNA sequencing of stool samples, and serum bile acid profiles were quantified using LC-MS/MS. Alpha and beta diversity were assessed, and differential abundance of bacterial genera was evaluated using non-parametric tests with false discovery rate (FDR) correction. Correlations between differentially abundant genera and bile acid levels were examined by Spearman's rank correlation. Results: genera and DCA/LCA levels. Discussion: may represent a candidate for next-generation probiotic development, further mechanistic and clinical studies are required to validate its therapeutic potential.