Continuous Enzymatic Peracids Synthesis in Pickering Emulsions: Influence of Nanoparticles Modification, Aqueous Phase Composition and Operational Parameters

This study provides a foundation for the development of sustainable and efficient continuous processes for oxidative biotransformations using Pickering emulsions (PE). By enabling the enzymatic production of peracids, the first step in lipase-catalyzed oxidative functionalization, the process addresses key limitations of previously explored green reaction systems, such as enzyme deactivation and low specific activity. The PE system enhances emulsion stability and preserves enzyme activity under high oxidant concentrations, achieving high yields and space-time productivity in a membrane-based continuous reactor. Surface-modified silica nanoparticles further improve interfacial catalytic efficiency as well as emulsion stability. This approach is well-suited for selective and eco-friendly oxidation in the synthesis of fine chemicals, active pharmaceutical ingredients, and specialty materials. Additionally, the robustness of the system allows stable operation under harsh conditions, supporting the efficient integration of the second, chemical epoxidation step. These findings contribute to the broader implementation of continuous green chemistry technologies in industrial biocatalysis.