Vascular Sphingosine Kinase 1 Regulates Angiotensin II–Induced Hypertension

BACKGROUND: deletion exhibit alleviated AngII (angiotensin II)-induced HTN. However, cellular mechanisms underlying this protective effect remain unclear due to pleiotropic S1P actions. METHODS: in smooth muscle cells (SMCs) or endothelial cells were generated using the Cre-loxP system. To induce HTN, mice were infused with AngII (490 ng/min per kilogram for 14 days), using osmotic minipumps. Blood pressure was monitored, and vascular structure and function were assessed ex vivo using wire/pressure myography and histological analysis. To identify mechanistic pathways, RNA sequencing was performed on mouse aortas and mesenteric arteries, followed by Gene Set Enrichment Analysis and validation by RT-qPCR/Western blotting. RESULTS: mice. CONCLUSIONS: plays a predominant role in AngII-induced HTN by modulating myogenic tone.