Genome-Informed Identification of Species-Specific Diagnostic Markers for Listeria Using Pangenome Analysis
reference strains (ATCC 19117 and ATCC BAA-679) with conventional PCR and gel electrophoresis to confirm expected amplicon sizes and specificity. These findings establish a genome-informed, specificity-driven framework for marker development and highlight the accessory genome as a valuable source of diagnostic loci, supporting accurate detection, epidemiological surveillance, and food safety monitoring.
Authors
- Kingsley E. Bentum (ORCID: https://orcid.org/0000-0001-6292-8582)
- Rejoice Nyarku (ORCID: https://orcid.org/0000-0002-4412-8714)
- Viona Osei (ORCID: https://orcid.org/0000-0003-0710-6214)
- Woubit Abebe
- Emmanuel Kuufire
- Asmaa Elrefaey
- Tyric James
- Temesgen Samuel
Institutions
- University of Sadat City (EG)
- Tuskegee University (US)
Publication Details
- Journal
- Pathogens
- Published
- 2026-04-07
- DOI
- https://doi.org/10.3390/pathogens15040397
- Primary Topic
- Listeria monocytogenes in Food Safety
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- U.S. Department of Agriculture
- National Institutes of Health
- National Institute of Food and Agriculture