Dynamic Regulation Coupled with Metabolic Pathway Optimization Enables High-Efficiency Lacto- <i>N</i> -fucopentaose I Biosynthesis in <i>Escherichia coli</i>
as an effective exporter that enhanced LNFP I secretion. Strengthening carbon source utilization and optimizing the fermentation process enabled the development of a high-producing strain that achieved 3.50 g/L LNFP I in shake-flask fermentation and 68.10 g/L in a 5 L bioreactor, with an LNT-to-LNFP I conversion rate of 92.10% and a productivity of 0.87 g/L/h.
Authors
- Jinyong Wu
- Caiwen Lao
- Jin Wang
- Xiangsong Chen
- Lixia Yuan
- Jianming Yao
Institutions
- University of Science and Technology of China (CN)
- Chinese Academy of Sciences (CN)
- Hefei Institutes of Physical Science (CN)
- Czech Academy of Sciences, Institute of Plasma Physics (CZ)
- Kai Biotech (South Korea) (KR)
Publication Details
- Journal
- Journal of Agricultural and Food Chemistry
- Published
- 2026-04-07
- DOI
- https://doi.org/10.1021/acs.jafc.5c17016
- Primary Topic
- Infant Nutrition and Health
- Type
- article
- Field-Weighted Citation Impact
- 0.00