Balancing Consecutive Two-Step Oxidations Catalyzed by a Single Cytochrome P450 Enables Efficient Biosynthesis of Plant Natural Products
OH to R-COOH. Consequently, the product, betulinic acid, was greatly improved in an engineered yeast strain, substantially reducing the accumulation of intermediates. Thus, this work establishes a mechanism-driven strategy to rebalance consecutive two-step oxidations, providing a potential strategy for the efficient biosynthesis of other PNPs that involve P450-catalyzed consecutive two-step oxidations.
Authors
- Y. Manjula Rao
- Zhenbo Yuan (ORCID: https://orcid.org/0000-0002-2435-0318)
- Zhengshan Luo (ORCID: https://orcid.org/0000-0002-3087-5663)
- Changmei Liu
- Yan Zhang
- Yilin Chen
Institutions
- Jiangnan University (CN)
Publication Details
- Journal
- ACS Synthetic Biology
- Published
- 2026-04-07
- DOI
- https://doi.org/10.1021/acssynbio.6c00016
- Primary Topic
- Plant Gene Expression Analysis
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Jiangnan University
- Science and Technology Support Program of Jiangsu Province
- Fundamental Research Funds for the Central Universities
- Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education