Digital and Catalytic Biosensing: Leveraging DNAzyme and Droplet Microfluidics for Ultrasensitive and High-Throughput Pathogen Detection

The integration of DNAzyme and droplet-based microfluidics is advancing pathogen detection toward higher sensitivity and throughput. However, this interdisciplinary convergence still faces multiple technical challenges. Based on practical applications, this article systematically outlines current key bottlenecks. It also proposes corresponding strategies and envisions future pathways for intelligent and precision-oriented integration.