Phase separation of C19orf12 regulates BNIP3 protein quality control and maintains neuronal mitophagy
; BNIP3: BCL2 interacting protein 3; BTZ: bortezomib; C19orf12: chromosome 19 open reading frame 12; CFP: cyan fluorescent protein; CHX: cycloheximide; DNP: dinitrophenyl; FCCP: carbonyl cyanide-p-trifluoromethoxyphenylhydrazone; FRAP: fluorescence recovery after photobleaching; GFP: green fluorescent protein; H&E stain: haematoxylin and eosin stain; LCD: low complexity domain; LC/MS: liquid chromatography-mass spectrometry; LLPS: liquid-liquid phase seperation; MAP1LC3B/LC3B: microtubule-associated protein 1 light chain 3 beta; mito-SRAI: mitochondrial signal-retaining autophagy indicator; MG132: cbz-leu-leu-leucinal; MMP: mitochondrial membrane potential; MPAN: mitochondrial membrane protein-associated neurodegeneration; NBIA: neurodegeneration with brain iron accumulation;PLA: proximity ligation assay; RFP: red fluorescent protein; ROS: reactive oxygen species; STX17: syntaxin 17; TFAM: transcription factor A, mitochondrial; TOLLES: TOLerance of lysosomal EnvironmentS; YPet: YFP for energy transfer.
Authors
- Sabina Bhatta
- Sandra L. Siedlak (ORCID: https://orcid.org/0000-0002-0393-4883)
- Meera Kumari
- Sandy Torres (ORCID: https://orcid.org/0000-0001-8602-6057)
- Fanpeng Zhao (ORCID: https://orcid.org/0000-0001-6441-7938)
- Changjuan Shao (ORCID: https://orcid.org/0000-0002-5417-719X)
- Wenzhang Wang
- Xiongwei Zhu
- Fengqin Wu
Institutions
- Case Western Reserve University (US)
Publication Details
- Journal
- Autophagy
- Published
- 2026-04-06
- DOI
- https://doi.org/10.1080/15548627.2026.2655834
- Primary Topic
- Neurological diseases and metabolism
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Institutes of Health