A REVIEW FOR METHOD DEVELOPMENT AND VALIDATION PARAMETERS BY USING HPLC TO DIFFERENT PHARMACEUTICAL DOSAGE FORMS

The techniques through which the chemical components present in complex mixtures are Separated, identified, and determined is termed chromatography. This technique is widely used like spectroscopy and is a very powerful tool not only for analytical methods but also for preparative methods. Compounds of high-grade purity can be obtained by this method. Chromatography can be simply as follows: “It is the technique in which the components of a mixture are separated based upon the rates at which they are carried or moved through a stationary phase (column) by a gaseous or liquid mobile phase”.