Protocol for stable isotope tracing of primary human peripheral blood mononuclear cells
C glutamine. We then detail procedures for derivatization, metabolite purification, and tracing analysis via single-quadrupole gas chromatography-mass spectrometry (GC-MS).
Authors
- Barbara S. Nikolajczyk (ORCID: https://orcid.org/0000-0001-5647-5740)
- William B. Inabnet (ORCID: https://orcid.org/0000-0002-1010-2065)
- Holden C. Williams
- Philip A. Kern (ORCID: https://orcid.org/0000-0002-4761-7417)
- Cecily R. Wood (ORCID: https://orcid.org/0000-0003-1259-3862)
- Joshua P. Steiner
- Varun Jain
- Samantha N. Hart
- Lance A. Johnson
Institutions
- University of Kentucky (US)
Publication Details
- Journal
- STAR Protocols
- Published
- 2026-04-07
- DOI
- https://doi.org/10.1016/j.xpro.2026.104450
- Primary Topic
- Isotope Analysis in Ecology
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Institutes of Health
- Diabetes Research Center, University of Washington
- NIH Office of the Director
- National Institute of General Medical Sciences
- National Institute of Diabetes and Digestive and Kidney Diseases