Bacterial extracellular vesicles as recyclable nutrient reservoirs
Bacterial extracellular vesicles (EVs) are known to mediate intercellular communication, virulence, and immune modulation. Here we show that bacteria can utilise EVs also as recyclable nutrient reservoirs. Using Bacillus cereus as a model organism, we demonstrate that EVs exhibit distinct dynamics depending on growth conditions: EVs produced in complex nutrient-rich media undergo time-dependent degradation, while those produced in defined nutrient-limited conditions remain stable and accumulate. We observe similar EV degradation patterns in Staphylococcus aureus. Time-resolved multi-omics profiling reveals that EVs containing the lipid sphingomyelin undergo progressive degradation. Using pharmacological inhibition, knockout mutants, and enzymatic complementation, we show that this process is driven by secreted sphingomyelinase (SMase). This enzyme contributes to degradation of sphingomyelin-containing EVs, thereby releasing their biomolecular cargo which can be used as a nutrient source. Growth assays confirm that SMase-mediated EV degradation provides a growth advantage when nutrients become depleted, thus establishing EVs as dynamic nutrient reservoirs.
Authors
- Waltraud Tschulenk
- Dr Monika Ehling-Schulz
- Benedikt Kirchner (ORCID: https://orcid.org/0000-0003-3878-0148)
- Mia S. C. Yu (ORCID: https://orcid.org/0000-0003-4141-6443)
- Masoumeh Alinaghi (ORCID: https://orcid.org/0000-0001-9304-1239)
- Michael W. Pfaffl (ORCID: https://orcid.org/0000-0002-3192-1019)
- Dapi Menglin Chiang
- Christina Ludwig (ORCID: https://orcid.org/0000-0002-6131-7322)
- Astrid Digruber (ORCID: https://orcid.org/0000-0002-8809-2530)
- Tanja V. Edelbacher (ORCID: https://orcid.org/0009-0004-6640-0346)
- Susanne I. Wudy (ORCID: https://orcid.org/0009-0001-6333-5233)
- Ingrid Walter
- Jan Přibyl
- Stefan Kummer
Institutions
- University of Veterinary Medicine Vienna (AT)
- University of Basel (CH)
- Masaryk University (CZ)
- University Medical Center Utrecht (NL)
- LMU Klinikum (DE)
- Central European Institute of Technology – Masaryk University (CZ)
- Technical University of Munich (DE)
- Ludwig-Maximilians-Universität München (DE)
Publication Details
- Journal
- Nature Communications
- Published
- 2026-04-06
- DOI
- https://doi.org/10.1038/s41467-026-71463-3
- Primary Topic
- Bacterial Infections and Vaccines
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Deutsche Forschungsgemeinschaft
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
- OeAD-GmbH
- Hochschuljubiläumsstiftung der Stadt Wien
- Erasmus+