19F NMR Strategy for Probing Site-specific RNA Dynamics in Large RNA-protein Complexes: Application to the Hfq RNA Chaperone
F Carr-Purcell-Meiboom-Gill (CPMG) relaxation dispersion experiments reveal specific changes in RNA dynamics on different surfaces of Hfq. Our NMR strategy provides a framework for studying how RNA-binding proteins like Hfq selectively alter the conformational dynamics of their RNA targets.
Authors
- Christoph Kreutz (ORCID: https://orcid.org/0000-0002-7018-9326)
- Ananya Majumdar (ORCID: https://orcid.org/0000-0001-9899-0433)
- Elise N White (ORCID: https://orcid.org/0000-0002-1299-9646)
- David Glänzer
- Sarah A. Woodson
- T.Kwaku Dayie
Institutions
- Johns Hopkins University (US)
- Universität Innsbruck (AT)
- University of Maryland, College Park (US)
Publication Details
- Journal
- Journal of Molecular Biology
- Published
- 2026-04-06
- DOI
- https://doi.org/10.1016/j.jmb.2026.169797
- Primary Topic
- RNA and protein synthesis mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Austrian Science Fund
- Österreichische Forschungsförderungsgesellschaft
- National Institute of General Medical Sciences
- National Institute of Allergy and Infectious Diseases
- Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases