A Constrained Degron Tag Engineered to Enhance Auxin-Inducible Protein Degradation
. Inserting mIAA7 into GFP, rather than fusing it directly at the GFP terminus, elevated expression of the GFP fusion protein and enabled more complete degradation upon auxin induction. The utility of the novel cAID tag was further validated by tagging a soluble cytosolic anti-mCherry nanobody and by targeting β-carotene ketolase, squalene synthase, and squalene epoxidase to modulate carotenoid biosynthesis. Compared to direct fusion with the conventional AID tag, cAID tag enabled more complete protein degradation and tighter temporal control of metabolism, while minimizing perturbation to the tagged protein.
Authors
- Zhenlin Han (ORCID: https://orcid.org/0000-0003-4391-6134)
- Jagdish Suresh Patel (ORCID: https://orcid.org/0000-0003-4999-5347)
- Jessica Maruwan
- Solange Tofani (ORCID: https://orcid.org/0009-0007-9075-3637)
- Adham M. Alkhadrawi
- Wanting Su
- L. América Chi
Institutions
- University of Hawaiʻi at Mānoa (US)
- University of Idaho (US)
- Key Laboratory of Guangdong Province (CN)
- Taiyuan Heavy Industry (China) (CN)
- Campbell Collaboration (NO)
Publication Details
- Journal
- ACS Synthetic Biology
- Published
- 2026-04-06
- DOI
- https://doi.org/10.1021/acssynbio.5c00970
- Primary Topic
- Protein Degradation and Inhibitors
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- University of Hawai'i
- National Institute of Food and Agriculture
- National Institute of General Medical Sciences