Abstract 956: The repurpose of dabigatran etexilate for improving prostate cancer prevention and treatment through down-regulating Myc and E2F target genes and inducing autophagic apoptosis

Abstract Dabigatran etexilate (DAB), an FDA-approved oral anticoagulant drug, has been safely used in cancer patients. While it has been concurrently administered with anti-androgens like enzalutamide in some prostate cancer patients, the potential interactions between DAB and enzalutamide and their effects on prostate cancer cells remain unknown. In this study, we have shown that DAB selectively inhibited prostate cancer cell growth with minimal growth inhibitory effects on normal prostate epithelial cells. Dabigatran induced autophagic apoptosis as evidenced by LC3B/Caspase 3 cleavages and increased autophagic vesicles. Oral administration of DAB significantly inhibited in vivo tumor growth in a xenograft model of 22Rv1 cells and reduced AR and Ki67 expression levels in tumor tissues. A systematic transcriptome analysis of gene expression regulation in DAB treated 22Rv1 cells revealed that the DAB regulated genes were significantly enriched in c-Myc and E2F targets and apoptosis and G2M checkpoint related genes. In addition, DAB acted synergistically with enzalutamide to reduce cell viabilities of prostate cancer cell lines: 22Rv1 and MyC-CaP. Our results indicate that DAB deserves further investigation as a novel anti-cancer agent, for prostate cancer prevention and for treatment of prostate cancer in combination with the main clinically used drug enzalutamide. Citation Format: Guanxing Zhai, Vinh Le, Liankun Song, Yixi “Michael” Wu, Xiaolin Zi. The repurpose of dabigatran etexilate for improving prostate cancer prevention and treatment through down-regulating Myc and E2F target genes and inducing autophagic apoptosis [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2026; Part 1 (Regular Abstracts); 2026 Apr 17-22; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2026;86(7 Suppl):Abstract nr 956.