Double-Stranded Universal Probe Strategy Enables Cost-Effective and Versatile Nucleic Acid Amplification Tests
copies/μL), with a limit of detection ranging from 10 copies/μL to 100 copies/μL. Furthermore, results from clinical samples showed high consistency and concordance with the gold standard qPCR, confirming the clinical viability and reliability of the ds-Uprobe-mediated assays. This simple, cost-effective, and highly versatile strategy enables sensitive and accurate detection and holds substantial promise for broader integration into various nucleic acid amplification tests for molecular diagnostics.
Authors
- Xinhua Yu (ORCID: https://orcid.org/0000-0003-3014-3435)
- Binhua Dong (ORCID: https://orcid.org/0000-0002-5086-0696)
- Qiuyuan Lin (ORCID: https://orcid.org/0000-0002-6809-5611)
- Guopeng Teng (ORCID: https://orcid.org/0009-0003-0957-1571)
- Yilin Zheng (ORCID: https://orcid.org/0000-0003-0627-3360)
- G.F. Lin (ORCID: https://orcid.org/0000-0002-4828-8985)
- Qingquan Chen
- Heng Xue
- Jingyi Chen (ORCID: https://orcid.org/0009-0004-6576-1086)
- Rong Wang
- Tianyao Xie
Institutions
- Fujian Medical University (CN)
- Fuzhou Maternity and Child Health Care Hospital (CN)
- Taiwan Centers for Disease Control (TW)
Publication Details
- Journal
- Analytical Chemistry
- Published
- 2026-04-03
- DOI
- https://doi.org/10.1021/acs.analchem.5c07993
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Fujian Provincial Finance Department
- National Natural Science Foundation of China
- Natural Science Foundation of Fujian Province
- Fujian Medical University