How long should washout be to reliably measure hepatic de novo lipogenesis using deuterated water? Exploratory analysis from a randomized crossover trial
Repeated deuterated water dosing in humans does not systematically bias measures of fasting hepatic DNL when using a washout duration of ∼4 wk. Fasting fractional hepatic DNL can be reliably assessed using deuterated water over sequential visits with as little as 3 wk washout between measures. At the group level, the within-participant variability in fasting hepatic DNL was relatively small and permits efficient detection of minimal clinically important differences in hepatic DNL with crossover studies.
Authors
- JENNIFER L. MAHER (ORCID: https://orcid.org/0000-0001-6603-536X)
- Françoise Koumanov (ORCID: https://orcid.org/0000-0002-9029-3390)
- Louise Bradshaw (ORCID: https://orcid.org/0000-0002-4201-0819)
- Leanne Hodson (ORCID: https://orcid.org/0000-0002-2648-6526)
- ALFONSO MORENO-CABAÑAS
- D. Thompson
- Elspeth Johnson (ORCID: https://orcid.org/0000-0003-4173-7837)
- James A. Betts (ORCID: https://orcid.org/0000-0002-9129-5777)
- Kaitlyn MJH Dennis
- Emma E. Vincent (ORCID: https://orcid.org/0000-0002-8917-7384)
- Steven Carter (ORCID: https://orcid.org/0000-0001-9810-0138)
- Javier T. Gonzalez
- Bruno Spellanzon
- Coco A. Taylor Start
Institutions
- University of Bristol (GB)
- University of Oxford (GB)
- University of Bath (GB)
- University of Castilla-La Mancha (ES)
Publication Details
- Journal
- American Journal of Physiology-Endocrinology and Metabolism
- Published
- 2026-04-03
- DOI
- https://doi.org/10.1152/ajpendo.00074.2026
- Primary Topic
- Diet and metabolism studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- British Heart Foundation