Comparison of the Antimicrobial Properties of Nanoemulsions Made from Phytosphingosine and Chitosan with a Triple Antibiotic Paste Against Enterococcus faecalis in Endodontics: An in-vitro Study

Purpose: in endodontics. Patients and Methods: Assuming the Minimum Inhibitory Concentration (MIC) of 15.93 and SD 1.53 with estimated error of 1, 80% power, and 5% alpha error, the sample size was calculated as 9. All three ICMs were prepared following the standard protocols. These ICMs were categorised into 3 groups as follows: Group I: PHSN; Group II: CHN; Group III: TAP. The antibacterial property of these ICMs was evaluated using MIC and disk diffusion methods. ANOVA and post hoc Tukey's tests. Results: . PHSN showed the largest zone of inhibition (38.3 ± 1.48), followed by CHN (8.16 ± 0.966), and TAP (6.30 ± 1.71). Thus, PHSN showed significantly higher antimicrobial efficiency compared to CHN and TAP (p < 0.05). Although CHN showed a higher zone of inhibition than the TAP, the difference between them was statistically nonsignificant. Conclusion: , and it can hinder its growth with a minimum inhibitory concentration of 1μg/mL. Chitosan nano-emulsion (CHN) shows slightly more efficacy than Triple Antibiotic Paste (TAP), but without any statistical significance.