Formulation Screening of Lipid Nanoparticles Enhances mRNA Delivery to Retina
, LNP B11 mediated mRNA expression localized to the retinal pigment epithelium, as demonstrated by mCherry expression. Furthermore, Cre mRNA delivery using B11 formulation induced tdTomato activation in Ai14 reporter mice more effectively than standard SM-102 and MC3 formulations, indicating that B11 supports functional protein expression in retinal tissue. Together, these findings identify B11 as a promising optimized LNP formulation for retinal mRNA delivery, highlighting the critical role of systematic formulation optimization in advancing nucleic acid therapeutics for retinal diseases.
Authors
- Li Zhou (ORCID: https://orcid.org/0000-0002-5851-7534)
- ChuanDong Cheng
- Qingjun Zhu (ORCID: https://orcid.org/0000-0001-6473-2153)
- Jinghua Yu (ORCID: https://orcid.org/0000-0001-5043-0322)
- Baoqing Li (ORCID: https://orcid.org/0000-0003-1679-8718)
- Pei Lv
- Zesen Ma
- Changlin Tian
- Dongsheng Li
- Haiyang Tong
- Yuanyuan Li
Institutions
- University of Science and Technology of China (CN)
- Anhui University (CN)
- Chinese Academy of Sciences (CN)
- High Magnetic Field Laboratory (CN)
- Anhui Provincial Hospital (CN)
- First Affiliated Hospital of University of South China (CN)
- Beijing Academy of Science and Technology (CN)
- University of South China (CN)
Publication Details
- Journal
- Molecular Pharmaceutics
- Published
- 2026-04-03
- DOI
- https://doi.org/10.1021/acs.molpharmaceut.5c01760
- Primary Topic
- RNA Interference and Gene Delivery
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Hefei Science Center, Chinese Academy of Sciences
- National Key Research and Development Program of China
- Major Science and Technology Projects in Anhui Province