Space biomedicine‐oriented bioink and biomimetic blood‒brain barrier

Abstract During launch phase, hypergravity and vibration can potentially damage cell models used in space medical research. This will compromise the success of subsequent biomedicine research. Bioinks and bioprinting play a pivotal role in the successful delivery of cell models and the fabrication of tissue models for space biomedicine research. In order to streamline the process and conserve valuable resources, it is crucial to examine the storability of bioink and high survival human cells under space conditions. This study investigated the effects of hypergravity and vibration on blood‒brain barrier (BBB)‐related cells cultured in two‐dimensional (2D) monolayers and three‐dimensional (3D) decellularized matrices. Subsequently, cell proliferation, viability, and gene expression were assessed. Present study confirmed 3D embedded cells showed marked tolerance to launch‐associated mechanical factors, compared with the disruption on 2D adherent cells. In addition, the present study provided methods for storing hybrid bioink (decellularized extracellular matrix of mammalian soft tissue) and high viability of cells loaded in bioink under the existing storage conditions of space station. Printability, rheological property and ultrastructure of bioink is maintained after storage in 4°C for 4 weeks. Three types of BBB‐related cells can maintain their viability and function under the cryopreservation of preservatives. This study verified the feasibility of storing bioink and cells in orbit under low‐temperature conditions. It addresses the challenges associated with maintaining cell viability, limited experimental window, and susceptibility to launch processes in space‐based cell experiments. These findings provide a foundation for efficient and reproducible bioprinting in space.