Comprehensive evaluation of sacchachitosan-based transdermal films for wound care: Physical, chemical, and biological parameters

respectively, while degradation ranged from 52.3% to 81.9% under physiological conditions. Bioadhesive strength reached 79 g in higher concentration of SNPs formulations. The results of cytocompatibility demonstrated that >70% cell viability in optimal films (SF1-SF4), while excessive AgNPs concentration (SF5-SF7) decreased viability to below 30%, suggesting dose-dependent cytotoxicity. Hemolysis remained within acceptable limits in lower-concentration formulations. Mushroom produced sacchachitosan was found to exhibit better hydrophilicity (contact angle 40°) than traditional chitosan, higher antimicrobial activity and superior film stability. These findings highlight sacchachitosan-based nanocomposite films as promising sustainable biomaterials for advanced wound management, while emphasizing the need for nanoparticle optimization to balance antimicrobial efficacy and biocompatibility.