High‐Frequency Quantitative Ultrasound Parameters Correlate With Microstructural Properties in a Murine Collagenase Model of Patellar Tendinopathy

, and thickness between control and collagenase-injected groups (p < 0.05 for all). ESD and thickness correlated with histological scores (R = 0.50, p < 0.001 for both), and receiver operating characteristic analyses showed high classification accuracy (AUC = 0.82-0.89). These findings indicate that QUS parameters are sensitive to pathological alterations in tendon microstructure and may serve as surrogate markers of structural integrity. As a rapid, cost-effective, and portable imaging modality, QUS holds strong potential for longitudinal monitoring of tendinopathy and evaluating treatment response in both animal models and clinical settings. Statement of Clinical Significance: QUS parameters detected tendon microstructural changes, indicating potential for non-invasive longitudinal monitoring, although limited here to post-mortem imaging.