Lost in Projection? Gaussian Filtering Recovers Hidden Conformational States

), which highlights low-energy regions corresponding to conformational states. The accurate definition of these states, however, is often impeded by projection artifacts, resulting in artificially shortened state lifetimes or even the complete disappearance of states from the analysis. As demonstrated for a two-dimensional toy model, Gaussian low-pass filtering of the high-dimensional feature trajectory can restore the underlying free energy landscape, allowing recovery of previously hidden states. When applied to an all-atom folding trajectory of HP35, the number of microstates increases by an order of magnitude, which leads to metastable states that are long-lived and much better defined structurally, even compared to dynamically cored state trajectories.