A bioactive magnesium alloy scaffold integrated with BMSCs-Loaded 3D microspheres synergistically promotes femoral head osteonecrosis repair by improving the osteogenic-angiogenic microenvironment

ions to participate in microenvironmental regulation. The BMSCs-loaded 3D microspheres were firmly integrated with the scaffold, which increased BMSCs loading capacity and provided a protective growth environment that shielded cells from mechanical damage. Further experiments indicated that the composite system modulated the interactions among BMSCs, osteoblasts, and vascular endothelial cells. By activating Extracellular Matrix organization, Focal adhesion, and the PI3K-Akt signaling pathways, it regulated the osteoblast and endothelial cell-related bone-vascular microenvironment to promote femoral head repair. This study demonstrates that combining a bioactive magnesium scaffold with BMSCs-loaded microspheres synergistically promotes femoral head repair, highlighting the therapeutic potential of integrating bioactive ion release with cellular paracrine regulation.