How to collect immature oocytes from small antral follicles for the Saigon biphasic in vitro maturation protocol

<h2>Abstract</h2><h3>Objective</h3> To provide a detailed description of the clinical procedure for aspirating small antral follicles (2.0–8.0 mm) as part of the Saigon Biphasic in-vitro maturation (IVM) protocol. This includes indications for the procedure, patient and equipment preparation, the aspiration process, laboratory techniques for identifying cumulus-oocyte complexes (COCs), and the timing for embryo transfer. <h3>Design</h3> This instructional video presents real-time footage of clinical procedures and embryology practices. It offers step-by-step guidance and practical recommendations for conducting a gonadotrophin-free biphasic IVM cycle, also known as CAPA-IVM (Capacitation-IVM). <h3>Setting</h3> A tertiary in-vitro fertilization clinic. <h3>Patient(s)</h3> Patients undergoing IVM treatment. All individuals featured in the video have provided informed consent for its publication and online distribution, including on social media, journal websites, scientific platforms, and other relevant platforms. <h3>Exposure</h3> The video includes: <h3>Main Outcome Measure(s)</h3> Clinical techniques for immature oocyte retrieval and compact COCs identification for gonadotrophin-free CAPA-IVM protocol. <h3>Results</h3> Numerous viable COCs can be successfully retrieved using the oocyte retrieval techniques outlined for gonadotrophin-free CAPA-IVM. The video demonstrates an efficient and flexible approach to identifying compact COCs, adaptable to various clinical conditions. Embryo transfer may subsequently be performed as either a fresh or frozen cycle, depending on the patient's choice. <h3>Conclusion</h3> (s): IVM is a safe and effective treatment option for eligible patients, particularly those with high ovarian reserve or polycystic ovary syndrome (PCOS). This technique enables the retrieval of multiple immature COCs without ovarian stimulation. The procedures outlined may differ from those used in other centers, offering a standardized approach for collecting COCs from small follicles for IVF and gamete banking. The Saigon protocol, which combines gonadotrophin-free CAPA IVM with fresh embryo transfer, underscores the clinical potential of IVM as a practical alternative to conventional stimulation protocols.